Thursday, February 15, 2001, Midterm Exam #2, Microbiology 509

Name: KEY, NOT-TO-LEAVE-ROOM (YET), KEY, NOT-TO-LEAVE-ROOM (YET), KEY

(1) Whooping cough is caused by __________ (give complete binomial)

A: Bordetella pertussis (Chapter 9)

 

(2) Which of the following two specifically involve the binding of something to the bacteria being identified?

(i)                   Biochemical assays

(ii)                 Differential plating

(iii)                Immunological reactions

(iv)                Phage typing

(v)                 Total cell count determination

A: (iii) Immunological reactions; (iv) Phage typing (Chapter 9)

(3) Which of the following is not associated with (and is not) a fungus?

(i) Athlete's foot

(ii) Beer

(iii) Candida

(iv) Histoplasmosis

(v) Pinworm

A: (v) Pinworm (this actually is a worm) (Chapter 11)

(4) Which does not describe at least some protists?

(i) Chemoautotroph

(ii) Chemoheterotroph

(iii) Photoautotroph

(iv) Sporozoans

(v) Unicellular

A: (i) Chemoautotroph (only certain bacteria are chemoautotrophs) (Chapter 11)

(5) What is the Gram-staining characteristic of the cause of Peptic Ulcer Disease?

A: Gram-negative (Chapter 9)

(6) What is lysogenic conversion?

A: lysogenic conversion is a change in host phenotype upon acquisition of a prophage (Chapter 10)

 

(7) What is a mesophile?

A: a mesophile is an organism whose growth-rate optimum is 20 C to 40 C

 

(8) What equipment must one employ to do a direct bacterial count?

A: microscope (Chapter 6)

(9) What dose the word "clonal" mean in the context of monoclonal antibody?

A: Clonal means that the cells used to produce the antibodies were propagated from a single cell (asexually; no meiosis) (Chapter 8)

 

(10) Under what circumstances would you expect a bacterium to display plasmolysis?

A: when suspended in a hypertonic environment; lot's of solutes, particularly more than are found in the cytoplasm of the bacterium (Chapter 6)

(11) Transduction involves DNA packaged within a(n) __________.

A: Virus; bacteriophage; phage (Chapter 8)

(12) Three common ways to culture viruses entail growth on whole animals, employing embryonated eggs, and in __________.

A: tissue culture (Chapter 10)

(13) The standard strain of a given organism, the one to which other organisms are compared in the course of their identification, is termed the __________ strain, and is often the first strain of a given species that was isolated.

A: Type (Chapter 9)

(14) The process by which DNA is copied to produce RNA is called __________.

(i) Replication

(ii) Respiration

(iii) Reverse transcription

(iv) Transcription

(v) Translation

A: (iv) Transcription (Chapter 7)

(15) The Gram-positive cocci are notable for their tendency to induce pus formation but also, in contrast to members of genus Bacillus or genus Clostridium (which are rods), for their inability to produce __________.

A: endospores (Chapter 9)

(16) The envelope proteins called spikes are responsible for accomplishing what for the obligate intracellular parasites that display them?

(i) Adsorption

(ii) Exocytosis

(iii) Initiating sex

(iv) Regeneration

(v) Transformation

A: (i) Adsorption (Chapter 10)

(17) The adjectives polyhedral, helical, and complex describe what aspect of what?

A: The shape of a virus' capsid (Chapter 10)

 

(18) Plus or minus is used to describe a virus' _________ genome. (a type of macromolecule)

A: RNA (ssRNA) (Chapter 10)

(19) Obligate aerobes require what to make their ATP? (besides a sufficiently rich culture medium)

A: oxygen (molecular oxygen; O2) (Chapter 6)

(20) Matching: (a) cats (b) mosquitoes, (c) sex, (c) water, (e) yeast. (use each only once)

(i) Giardia

(ii) Plasmodium

(iii) Saccharomyces

(iv) Toxoplasma

(v) Trichomonas

A: (i) (c) water, (ii) (b) mosquitoes, (iii) (e) yeast, (iv) (a) cats, (v) (c) sex (Chapter 11)

(21) In the five-kingdom system of classification there are two kingdoms that consist predominantly of unicellular organisms, members of kingdom Protista and also members of kingdom _________.

A: Monera (Chapter 9)

(22) In the course of the spread plate technique, what is it that is added to the solid medium? Pick all that apply

(i) Melted agar

(ii) Bacteria culture

(iii) Sterile broth

(iv) An inoculating needle

(v) Distilled water

A: (ii) Bacteria culture (Chapter 6)

(23) In PCR, how does one treat the DNA in order to unwind the double helix? (that is, separate the two DNA strands making up the double helix)

(i) Aggitation

(ii) DNA gyrase

(iii) DNA polymerase

(iv) Heat

(v) Reverse transcriptase

A: (iv) Heat (Chapter 8)

(24) In bacteria, what step serves to complete the sexual act?

(i) Cell division

(ii) Recombination

(iii) Regeneration

(iv) Smoking a pilus

(v) Transformation

A: (ii) Recombination (of the "snippet" into the recipient cell's chromosome) (Chapter 8)

(25) How is it that bacterial chromosomes do not possess ends?

A: Bacterial chromosomes are circular so don't possess ends (Chapter 7)

(26) Helicobacter employs a(n)  _________ to effect motility.

A: flagellum/flagella (Chapter 9)

(27) Fungi by and large are saprophytes. What is a saprophyte.

A: A saprophyte is an organism that obtains its nutrients by consuming dead organisms (Chapter 11)

 

(28) From what kind of environments might you expect to isolate bacteria containing extremozymes?

                (i) Cow intestine

                (ii) Deep lakes

                (iii) Hot spring

                (iv) Sewage-treatment plant

                (v) Stratosphere

A: (iii) Hot spring (Chapter 9)

(29) Flukes and tapeworms are examples of

(i) Flatworms

(ii) Pinworms

(iii) Ringworms

(iv) Roundworms

(v) Segmented worms

A: (i) Flatworms (Chapter 11)

(30) During decline phase, if 90 out of 100 bacteria die in two hours, then what fraction of those original 100 bacteria will still be alive after a total of four hours of decline phase (that is, start the clock with 100 bacteria and tell me  what fraction of those bacteria will still be alive four hours later).

(i) One-half

(ii) 3 percent

(iii) 10

(iv) 1/100

(v) 0.01%

A: (iii) 1/100 (Chapter 6)

(31) Distinguish phage l from phage T4.

A: l is temperate while T4 is virulent (Chapter 10)

 

(32) Can an organism simultaneously serve as a mechanical vector and as a definitive host? Why or why not?

A: No, a definitive host is the host upon which a parasite goes through its sexual cycle, and this means that the parasite is not simply being transported on the organism, as the phrase mechanical vector implies (Chapter 11)

 

(33) A heritable change in the nucleotide sequence of a gene is called a __________.

A: Mutation (Chapter 7)

(34) __________ is a genus of filamentous bacteria that are medically useful due their production of antibiotics.

A: Streptomyces (Chapter 9)

(35) __________ are regions of DNA that specify the synthesis of RNAs. (no, nucleolus is not the answer)

A: Genes (Chapter 7)