Supplement to Lab Topic 14: Protists and Fungi

[return to lab schedule] [home] [class schedule] [BioPort] [contents]

àPlease observe and draw all displayed specimensß

(keeping in mind that traditionally introductory biology labs require

memorization of specimens for identification at a later date).

Map of Stations

[Front of Room]

1A, 1B, 1C	2A, 2B, 2C	3A, 3B, 3C	4A, 4B, 4C	5A, 5B, 5C
6A, 6B, 6C	7A, 7B, 7C	8A, 8B, 8C	9A, 9B, 9C	10A, 10B, 10C
11A, 11B, 11C	12A, 12B, 12C	13A, 13B, 13C	14A, 14B, 14C	15A, 15B, 15C
16A, 16B, 16C	17A, 17B, 17C	18A, 18B, 18C	19A, 19B, 19C	20A, 20B, 20C

[Back of Room]

Boxes in above table refer to desks.

Materials should be arranged in the order presented in your lab text.

Please let us know if there is a discrepancy or other problems.

Please be aware that you don’t necessary have to begin at station 1.

Please pay attention to suggested microscope magnifications (below)

(too-high magnification can be as misleading as too-low magnification).

Specimen List

Page	Sect.	Section Title	Stat.	Specimen	State	Container	Scope	Comments
356	14.1A	Rhizopods (Amoebas)	1A	Amoeba proteus	Live	Concave (other?)	Compound	May not be able to find
356	14.1A	Foraminiferans (Forams)	1C	w.m. Z264	Slide	Prepared	Compound	Observe using low power
357	14.1A	Actinopods (Radiolarians)	2A	w.m. Z180	Slide	Prepared	Compound
357	14.1A	Protozoa that move using flagella	2C	Trypanosoma lewisi SM. Z264	Slide	Prepared	Compound	Use oil immersion (ask instructor if you do not have experience with immersion oil)
357	14.1A	Same as above	3A	Euglena gracilis	Live	Slide	Compound
359	14.1.A	Protozoa that move using cilia	3C	Paramecium caudatum	Live	Slide	Compound	These move quickly so may not be (or stay) in your field of view; make sure you use protoslow; the bio lab microscopes may not be up to seeing as much detail as you would like; try lowering condenser to increase depth of field
359	14.1.A	Same as above	hood	Wild Ciliate (perhaps etc.)	Live	Concave (other?)	Phase-contrast	Exhibition, hay infusion, please do not adjust without help (Lab Prep Person: Lab Instructor will take care of setting this up)
362	14.1.B	Dinoflagellates (Dinoflagellata)	4A	Dinoflagellates	Live	Slide	Compound	May not be able to find
364	14.1.B	Diatoms (Bacillariophyta)	5A	w.m. 29-6020 (B25W)	Slide	Prepared	Compound	Very gorgeous; sketch five or six especially not-round examples (i.e., different from example in text)
364	14.1.B	Same as above	5C	PB 115	Slide	Slide (instructor prepare)	Compound	Compare to specimens in prepared slide; how do they differ?
365	14.1.B	Brown Algae (Phaeophyta)	6	Sargassum, Chondrus crispus (Irish moss), Fucus, Laminaria	Preserved	Observe in dish	Dissecting
366	14.1.B	Red Algae (Rhodophyta)	7A	Coralline Algae, OTHER?	Preserved	Observe in dish	Dissecting	(Lab Prep Person: Do we have any other red-algae specimens?)
369	14.1.C	The Green Algae (Chlorophyta)	8A	Spirogyra	Live	Slide	Compound
369	14.1.C	Same as above	8C	Ulva (sea lettuce)	Preserved	Observe in dish	Dissecting	Note that these specimens can be folded upon themselves and may require some effort to view in entirety
369	14.1.C	Same as above	9A	Cladophora	Live	Watch glass or nothing	Dissecting
369	14.1.C	Same as above)	9C	Volvox globator	Live	Concave	Compound (Dissecting?)	The large dots inside the globes are “baby” colonies; the texture you see as you adjust the focus up and down are individual cells
369	14.1.C	Same as above	10A	Pandorina	Live	Concave	Compound
369	14.1.C	Same as above	10C	Codium	Preserved	Observe in dish	Dissecting
370	14.1.D	Fungus-like Protists—Slime molds	11A	Physarum polycephalum	Live	Petri dish	Dissecting	Need to be able to remove top in order to see past condensation
372	14.2.A	Division Zygomycota—Zygote fungi	12A	Rhizopus zygospores	Live	Petri dish	Dissecting
372	14.2.A	Same as above	12C	Pilobolus crystallinus	Live	Petri dish	Dissecting	Note that we were unable to locate any Pilobolus Spring, 2000
375	14.2.B	Division Ascomycota—Sac fungi	13A	Morchella PB 216a	Preserved	Dish (or should this be kept in jar?)	Dissecting
375	14.2.B	Same as above	13A	Bulgaria cup fungus PB 221	Preserved	Dish (or should this be kept in jar?)	Dissecting
375	14.2.B	Same as above	13B	Cup fungus Apothecium Peziza or related form 29-7980	Slide	Prepared	Compound	You need to use low power on this; see sketch on p. 382 that needs to be filled in with detail
377	14.2.C	Division Basidiomhycota—Club fungi	14A	Coprinus Pileus x.s. 18-16	Slide	Prepared	Dissecting
377	14.2.C	Same as above	14B	Puff Ball	Preserved	As presented	None
377	14.2.C	Same as above	14C	Mushrooms from store	Fresh	None	Dissecting
379	14.2.D	Division Deuteromycota—Imperfecti Fungi	15A	Penicilium notatum	Live	Petri dish	Dissecting	Please place the alcohol lamp, matches and tweezers on this station
379	14.2.D	Same as above	15C	Blue cheese	Live	Dish	Dissecting
380	14.2.E	Lichens	16	Lichen, ascocarp	Slide	Prepared	Compound

Choice of containers:

· Concave slide: Use a concave slide when a normal slide will crush the specimen (e.g., Volvox)

· Dish (observe in): Many specimens will need to be removed from their containers to observe; an appropriate dish will be supplied for pouring the specimen into; please use a dish for this purpose only if you are instructed to (Laboratory prep person: please supply dishes and label specimens that should or should not be observed in dishes)

· Dissection pan: Any dissections will be done in a dissection pan; you will obtain specimens for dissections from stations then bring those specimens, in a dissection an, back to your desk; if the specimen is presented to you in a dissection pan, then that specimen should be observed at the station but not extensively dissection (i.e., poking and otherwise manipulating is OK, but cutting, etc., is not)

· Petri dish: Normally a petri dish will be employed if that is what the specimen is supplied in

· Slide (microscope): Use a slide when the specimen is relative small and will be observed using a compound scope

· No container: Some items are suffiently large or are already mounted such that a container is not necessary

Choice of microscopes:

· Compound microscope: Use for observing relatively small specimens

· Dissecting (stereoscopic) microscope: Use for observing the fine structure of relatively large specimens

· Phase-contrast microscope: Use for observing especially living bacteria

· No microscope: Some items are sufficiently large or its fine structure sufficiently inaccessible that we will not employ microscopic observation