Supplement to Lab Topic 15: Plant Diversity I

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àPlease observe and draw all displayed specimensß

(keeping in mind that traditionally introductory biology labs require

memorization of specimens for identification at a later date).

Map of Stations

[Front of Room]

1A, 1B, 1C	2A, 2B, 2C	3A, 3B, 3C	4A, 4B, 4C	5A, 5B, 5C
6A, 6B, 6C	7A, 7B, 7C	8A, 8B, 8C	9A, 9B, 9C	10A, 10B, 10C
11A, 11B, 11C	12A, 12B, 12C	13A, 13B, 13C	14A, 14B, 14C	15A, 15B, 15C
16A, 16B, 16C	17A, 17B, 17C	18A, 18B, 18C	19A, 19B, 19C	20A, 20B, 20C

[Back of Room]

Boxes in above table refer to desks.

Materials should be arranged in the order presented in your lab text.

Please let us know if there is a discrepancy or other problems.

Please be aware that you don’t necessary have to begin at station 1.

Please pay attention to suggested microscope magnifications (below)

(too-high magnification can be as misleading as too-low magnification).

Specimen List

Page	Sect.	Section Title	Stat.	Specimen	State	Container	Scope	Comments
397	15.1.A	Division Bryophyta	1A	Moss	Living	Large culture dish	None
397	15.1.A	Same as above	1C	Antheridial head ( #B361B)	Slide	Prepared	Compound
397	15.1.A	Same as above	2A	Archegonial head ( #B363)	Slide	Prepared	Compound
397	15.1.A	Same as above	2C	Mnium life cycle (#PB383)	Preserved	Bottle	None	Don’t remove from bottle; don’t draw
399	15.1.B	Division Hepatophyta	3A	Marchantia	Living	None	None
399	15.1.B	Same as above	3B	Liverwort types (#PB350)	Preserved	None	None	Don’t remove from bottle; don’t draw; lab prep person: please do not put out liverwort life cycle sheet since it confusingly contradicts the lab text
399	15.1.B	Same as above	3C	Ricciocarpus, Marchantia, Porella, Anthoceros (#POM 16510)	Lucite	None	Dissecting or None	Don't draw
404	15.2.B	Division Pterophyta (formerly Division Psilophyta)	4A	Whiskfern	Living	None	None	Keep in mind that your text mostly discusses the regular ferns (below), not the whiskferns… you will become confused if you attempt to locate division Pterophyta anatomy while observing these plants
402	15.2.A	Division Lycophyta	5A	Lycopodium lucidulum (or equivalent)	Living	None	None
402	15.2.A	Same as above	5B	Lycopodium complanatar	Preserved	None	None	Don’t remove from bottle; don’t draw
402	15.2.A	Same as above	5C	Selaganella (resurrection plant)	Living	None	None	Don’t draw
404	15.2.B	Division Sphenophyta	6A	Equisetum hyemale	Living	None	None
404	15.2.B	Same as above	6C	Equisetum hyemale L. “common field horsetail”	Preserved	Herbarium Sheet	None	Please be very careful with these preserved specimens
404	15.2.B	Division Pterophyta	7A	Fern sorus	Preserved	None	Dissecting	Note sori; draw at level-2 zoom
404	15.2.B	Same as above	7C	Fern	Living	None	None	Don’t draw; sori may not yet be present on living fern leaves
406	15.2.C	Same as above	8A	Fern sporangia (#B431)	Slide	Prepared	Compound	Note that fern sori are bundles of these sporangia; view/draw at 100x power; see poster immediately behind table (i.e., table 13); note that the Fiddler fern leaf is not a fern sporangia (i.e., it is shown in the poster approximately real size… it is not microscopic)
406	15.2.C	Same as above	8C	Fern leaflet indusium (fern sorus cross section) (#B432a)	Slide	Prepared	Compound	Note that sori are bundles of sporangia; sketch with detail only down to the outlines of sporangia; view/draw at lower power
406	15.2.C	Same as above	9A	Fern prothallium archegonia (fern gametophyte) (#B407)	Slide	Prepared	Compound	View/draw at low power
406	15.2.C	Same as above	9C	Fern prothallium antheridia (fern gametophyte) (#B405)	Slide	Prepared	Compound	View/draw at 100x power; note antheridia (these are where the sperm come from)
406	15.2.C	Same as above	10A	Fern prothallium with young sporophyte (fern gametophyte with young sporophyte) (no number—Frey Scientific)	Slide	Prepared	Dissecting	Use 1x power to view; don’t draw
406	15.2.C	Same as above	10C	Ferm gametophyte	Living	Petri dish	Dissecting	Don’t draw
409	15.2.D	Fossils	11A	Stigmaria	mold???	None	None	=underground stem
409	15.2.D	Fossils	11C	Lepidodendron	mold	None	None	Don’t draw
409	15.2.D	Fossils	11C	Lepidodendron	cast	None	None	Don’t draw
409	15.2.D	Fossils	12A	Calamite (Sphenopsids?)	cast	None	None	Don’t draw
409	15.2.D	Fossils	12A	Calamite	crushed cast	None	None	Don’t draw
409	15.2.D	Fossils	12C	Fossil of extinct fern	???	None	None	Don’t draw

Choice of containers:

· Concave slide: Use a concave slide when a normal slide will crush the specimen (e.g., Volvox)

· Dish (observe in): Many specimens will need to be removed from their containers to observe; an appropriate dish will be supplied for pouring the specimen into; please use a dish for this purpose only if you are instructed to (Laboratory prep person: please supply dishes and label specimens that should or should not be observed in dishes)

· Dissection pan: Any dissections will be done in a dissection pan; you will obtain specimens for dissections from stations then bring those specimens, in a dissection an, back to your desk; if the specimen is presented to you in a dissection pan, then that specimen should be observed at the station but not extensively dissection (i.e., poking and otherwise manipulating is OK, but cutting, etc., is not)

· Petri dish: Normally a petri dish will be employed if that is what the specimen is supplied in

· Slide (microscope): Use a slide when the specimen is relative small and will be observed using a compound scope

· No container: Some items are suffiently large or are already mounted such that a container is not necessary

Choice of microscopes:

· Compound microscope: Use for observing relatively small specimens

· Dissecting (stereoscopic) microscope: Use for observing the fine structure of relatively large specimens

· Phase-contrast microscope: Use for observing especially living bacteria

· No microscope: Some items are sufficiently large or its fine structure sufficiently inaccessible that we will not employ microscopic observation